Disulfide-Sulfhydryl Interchange Studies on Myosin A
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چکیده
منابع مشابه
Purification and properties of an enzyme from beef liver which catalyzes sulfhydryl-disulfide interchange in proteins.
An enzyme which catalyzes sulfhydryl-disulfide interchange in proteins containing “incorrect” disulfide bonds has been isolated from beef liver microsomes. The purified form of this enzyme shows two bands upon electrophoresis in starch gels and polyacrylamide gels. The proteins represented by these bands are considered to be variants of the same protein because both are enzymically active after...
متن کاملSulfhydryl and Disulfide Groups of Proteins
1. Methods have been described for reducing protein S-S groups, for oxidizing protein SH groups, and for estimating protein S-S and SH groups. 2. It has been found necessary in estimating the cystine content of proteins by the Folin-Marenzi method to take into account any cysteine that may be present. 3. A method for estimating the cysteine content of proteins has been described. 4. With these ...
متن کاملParticipation of the unsymmetrical disulfide of coenzyme A and glutathione in an enzymatic sulfhydryl-disulfide interchange. I. Partial purification and properties of the bovine kidney enzyme.
In addition, it has been detected in most rat tissues studied. Its pH optimum is 8.2 and the equilibrium constant for the reaction is near unity at pH 6.9 and 25’. Michaelis constants for reduced glutathione and CoASSG are 3.3 x 10-4?d and 4.5 x 10-5b& respectively. The molecular weight of the enzyme, determined by gel filtration, is approximately 12,000. Several unsymmetrical disulfides contai...
متن کاملMechanism of action of pyruvate kinase. Role of sulfhydryl groups in catalytic activity as determined by disulfide interchange.
Evidence is presented that four sulfhydryl residues play a critical role in the activity of pyruvate kinase. Modification of the four most reactive sulfhydryl groups by 5,5’-dithiobis(Z-nitrobenzoate) leads to a disulfide interchange reaction in which a chemically modified but catalytically active enzyme is produced. This form, which contains 5-thio-Z-nitrobenzoate in disulfide linkage to cyste...
متن کاملDifferential analysis of sulfhydryl and disulfide groups of intact spores.
Blankenship, L. C. (Eastern Utilization Research and Development Division, Washington D.C.), and M. J. Pallansch. Differential analysis of sulfhydryl and disulfide groups of intact spores. J. Bacteriol. 92:1615-1617. 1966.-Fluorescence quenching of fluorescein mercuric acetate in alkaline medium (1 n NaOH) was found to be an accurate, sensitive method for differential analysis of sulfhydryl and...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1964
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)91400-6